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minute plasma membrane/protein isolation and cell fractionation kit  (Invent Biotechnologies)


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    Invent Biotechnologies minute plasma membrane/protein isolation and cell fractionation kit
    Minute Plasma Membrane/Protein Isolation And Cell Fractionation Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+fraction+kit/Minute+Plasma+Membrane%2FProtein+Isolation+and+Cell+Fractionation+Kit/custom%40sm-005%4042495521
    Average 96 stars, based on 657 article reviews
    minute plasma membrane/protein isolation and cell fractionation kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: GRP78 promotes rabies virus entry through interacting with viral receptors.
    Article Snippet: .. Isolation of cell plasma membrane proteins To detect proteins on the cell membrane, HEK293 cells and N2a cells’ plasma mem branes were extracted using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Transferrin Receptor Protein 1 Cooperates with mGluR2 To Mediate the Internalization of Rabies Virus and SARS-CoV-2
    Article Snippet: .. To detect the interaction between mGluR2-Flag and endogenous TfR1, HEK293 cells expressing mGluR2-Flag protein from two 15 cm dishes were used to extract cell plasma membrane by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung
    Article Snippet: .. To detect the expression of ACE2 on the diltiazem-treated or CACNA1C-silenced cell surface, two 10-cm dishes of Vero-E6 cells were used to extract plasma membrane proteins by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies) following the manufacturer’s instructions. ..

    Clinical Proteomics:

    Article Title: GRP78 promotes rabies virus entry through interacting with viral receptors.
    Article Snippet: .. Isolation of cell plasma membrane proteins To detect proteins on the cell membrane, HEK293 cells and N2a cells’ plasma mem branes were extracted using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Transferrin Receptor Protein 1 Cooperates with mGluR2 To Mediate the Internalization of Rabies Virus and SARS-CoV-2
    Article Snippet: .. To detect the interaction between mGluR2-Flag and endogenous TfR1, HEK293 cells expressing mGluR2-Flag protein from two 15 cm dishes were used to extract cell plasma membrane by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung
    Article Snippet: .. To detect the expression of ACE2 on the diltiazem-treated or CACNA1C-silenced cell surface, two 10-cm dishes of Vero-E6 cells were used to extract plasma membrane proteins by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies) following the manufacturer’s instructions. ..

    Membrane:

    Article Title: GRP78 promotes rabies virus entry through interacting with viral receptors.
    Article Snippet: .. Isolation of cell plasma membrane proteins To detect proteins on the cell membrane, HEK293 cells and N2a cells’ plasma mem branes were extracted using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Transferrin Receptor Protein 1 Cooperates with mGluR2 To Mediate the Internalization of Rabies Virus and SARS-CoV-2
    Article Snippet: .. To detect the interaction between mGluR2-Flag and endogenous TfR1, HEK293 cells expressing mGluR2-Flag protein from two 15 cm dishes were used to extract cell plasma membrane by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung
    Article Snippet: .. To detect the expression of ACE2 on the diltiazem-treated or CACNA1C-silenced cell surface, two 10-cm dishes of Vero-E6 cells were used to extract plasma membrane proteins by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies) following the manufacturer’s instructions. ..

    Expressing:

    Article Title: Transferrin Receptor Protein 1 Cooperates with mGluR2 To Mediate the Internalization of Rabies Virus and SARS-CoV-2
    Article Snippet: .. To detect the interaction between mGluR2-Flag and endogenous TfR1, HEK293 cells expressing mGluR2-Flag protein from two 15 cm dishes were used to extract cell plasma membrane by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies, SM-005). ..

    Article Title: Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung
    Article Snippet: .. To detect the expression of ACE2 on the diltiazem-treated or CACNA1C-silenced cell surface, two 10-cm dishes of Vero-E6 cells were used to extract plasma membrane proteins by using the Minute Plasma Membrane Protein Isolation and Cell Fraction Kit (Invent Biotechnologies) following the manufacturer’s instructions. ..



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    A. IHC staining for WEE1 in Human EAC tissue sections depicting strong cytosolic localization B. Quantification of the Cytoplasmic and Nuclear Signals from A using Cell Profiler C. Cell <t>fractionation</t> of FLO1 and OE33 EAC cell lines followed by western blot for WEE1, p84 (Nuclear marker) and ὰ-Tubulin (Cytoplasmic marker) D- Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (Blue) in EAC cell lines FLO1 and OE33, captured at 40X Magnification.E.MYC mRNA expression in WEE1 high Vs WEE1 low EAC tissue samples derived from TCGA and 4 different GEO datasets. F. Gene Set Enrichment Analysis (GSEA) of MYC target genes in WEE1 high Vs WEE1 low EAC samples G. WEE1 mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. H. MYC mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . I. Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (blue) in normal esophagus and gastroesophageal adenocarcinoma tissue sections in TMA, captured at 20x magnification. J- Quantification of fluorescence intensity from I K – Correlation analysis between WEE1 and MYC signal intensity
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    Image Search Results


    A. IHC staining for WEE1 in Human EAC tissue sections depicting strong cytosolic localization B. Quantification of the Cytoplasmic and Nuclear Signals from A using Cell Profiler C. Cell fractionation of FLO1 and OE33 EAC cell lines followed by western blot for WEE1, p84 (Nuclear marker) and ὰ-Tubulin (Cytoplasmic marker) D- Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (Blue) in EAC cell lines FLO1 and OE33, captured at 40X Magnification.E.MYC mRNA expression in WEE1 high Vs WEE1 low EAC tissue samples derived from TCGA and 4 different GEO datasets. F. Gene Set Enrichment Analysis (GSEA) of MYC target genes in WEE1 high Vs WEE1 low EAC samples G. WEE1 mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. H. MYC mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . I. Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (blue) in normal esophagus and gastroesophageal adenocarcinoma tissue sections in TMA, captured at 20x magnification. J- Quantification of fluorescence intensity from I K – Correlation analysis between WEE1 and MYC signal intensity

    Journal: Cancer letters

    Article Title: WEE1 Stabilizes MYC to Promote Therapeutic Resistance in Esophageal Adenocarcinoma

    doi: 10.1016/j.canlet.2026.218418

    Figure Lengend Snippet: A. IHC staining for WEE1 in Human EAC tissue sections depicting strong cytosolic localization B. Quantification of the Cytoplasmic and Nuclear Signals from A using Cell Profiler C. Cell fractionation of FLO1 and OE33 EAC cell lines followed by western blot for WEE1, p84 (Nuclear marker) and ὰ-Tubulin (Cytoplasmic marker) D- Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (Blue) in EAC cell lines FLO1 and OE33, captured at 40X Magnification.E.MYC mRNA expression in WEE1 high Vs WEE1 low EAC tissue samples derived from TCGA and 4 different GEO datasets. F. Gene Set Enrichment Analysis (GSEA) of MYC target genes in WEE1 high Vs WEE1 low EAC samples G. WEE1 mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. H. MYC mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . I. Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (blue) in normal esophagus and gastroesophageal adenocarcinoma tissue sections in TMA, captured at 20x magnification. J- Quantification of fluorescence intensity from I K – Correlation analysis between WEE1 and MYC signal intensity

    Article Snippet: Cell fractionation in FLO1 and OE33 cell lines was carried out according to the protocol described in the Cell Fractionation Kit (CST).

    Techniques: Immunohistochemistry, Cell Fractionation, Western Blot, Marker, Immunofluorescence, Staining, Expressing, Derivative Assay, Fluorescence